Dictionary > Gram-indeterminate bacteria

Gram-indeterminate bacteria

Definition
noun, singular: gram-indeterminate bacterium
Bacteria that do not respond to gram staining and, therefore, are indeterminate as either Gram-positive or Gram-negative
Supplement
Gram’s method or gram staining is a simple microbiological procedure that enables quick identification of bacterial species as either gram-positive or gram-negative. Bacterial cells that are violet in color from the primary staining (with crystal violet dye) under the microscope are called gram-positive. Bacterial cells that lose the violet color and take the counterstain color are called gram-negative bacteria. The differing color results in bacterial cells after gram staining are associated with the structural differences in bacterial cell’s outer covering. Gram-positive bacteria have a thick cell wall comprised of a lot of peptidoglycans and teichoic acids that enable them to retain the crystal violet colour. Gram-negative bacteria have less peptidoglycan. The crystal violet gram stain colour is easily lost from ethanol-alcohol mix during decolorization. Because of this, they can be stained with the safranin dye (pink) after counterstaining. There are certain bacteria that are not responding the same way to gram staining, such as acid fast bacteria. It is because they have a different type of cell envelope. The fatty acids in their cell wall resist gram staining. For this type of bacteria, Ziehl-Neelson staining method is used.
Compare:

See also:

  • gram staining


  • You will also like...

    Fossil trilobite imprint in the sediment
    Insects

    There are more species of insects than any other species combined. This surely illustrates that insects have the selecti..

    Growth Patterns
    Growth Patterns

    This tutorial describes the sigmoid curve, annual plant growth, tree growth, human growth, and insect growth as the grow..

    Freshwater Ecology
    Freshwater Ecology

    Freshwater ecology focuses on the relations of aquatic organisms to their freshwater habitats. There are two forms of co..

    sponge
    Primitive Animals

    Life, as we know it today, is presumed to have started in the sea and many of them were likely eukaryotic animal-like or..

    river
    Running Water Freshwater Communities

    This tutorial introduces flowing water communities, which bring new and dithering factors into the equation for possible..

    New Zealand Fauna
    New Zealand’s Unique Fauna

    Meet some of New Zealand's unique fauna, including endemic insects, frogs, reptiles, birds, and mammals, and investigate..

    Related Articles...

    No related articles found

    See all Related Topics